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pchk2 thr68 cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pchk2 thr68 cell signaling
    Pchk2 Thr68 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pchk2 thr68 cell signaling/product/Cell Signaling Technology Inc
    Average 96 stars, based on 793 article reviews
    pchk2 thr68 cell signaling - by Bioz Stars, 2026-03
    96/100 stars

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    Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 <t>(pCHK2)</t> was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells
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    Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 <t>(pCHK2)</t> was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells
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    Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 <t>(pCHK2)</t> was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells
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    Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 <t>(pCHK2)</t> was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells
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    Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 (pCHK2) was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells

    Journal: eLife

    Article Title: Synergistic effect of inhibiting CHK2 and DNA replication on cancer cell growth

    doi: 10.7554/elife.104718

    Figure Lengend Snippet: Figure 4. Isobavachalcone (IBC) inhibits the CHK2 kinase. (A) Selected protein kinases were incubated with the indicated range of IBC concentrations, and kinase activity in vitro was determined by using a radiometric assay. The IC50 of IBC for each kinase is indicated in the panel on the right. (B) MCF- 7 cells were pretreated with DMSO, 15 µM IBC, or 20 µM BML-277 for 2 hr, then camptothecin (CPT, 1 μM) was added for 2 hr. Phosphorylation of CHK2 on S516 (pCHK2) was detected by western blotting. The ratio of pCHK2-S516 induction, relative to the DMSO + CPT control, is indicated (n = 3). (C) MCF-7 cells were treated as indicated in (B). Phosphorylation of chromatin-bound BRCA1 at residue S988 was detected by western blotting. The relative ratio of pBRCA1-S988 signal, after normalization to Ponceau signal, is indicated. TBP was used as a marker of chromatin fraction. (D) MCF-7 cells

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) Immortalized BJ fibroblasts Dr. D. Peeper The Netherlands Cancer Institute, Amsterdam Foreskin (normal neonatal mal) Cell line (H. sapiens) MCF- 7 HTB- 22 ATCC Mammary gland adenocarcinoma Cell line (H. sapiens) A549 CRM- CCL- 185 ATCC Lung carcinoma Cell line (H. sapiens) HCC827 CRL- 2868 ATCC Lung adenocarcinoma Cell line (H. sapiens) PC3 CRL- 1435 ATCC Prostate adenocarcinoma Cell line (H. sapiens) DU145 HTB- 81 ATCC Prostate carcinoma Cell line (H. sapiens) U937 CRL- 1593.2 ATCC Histiocytic lymphoma Cell line (H. sapiens) HCT116 CCL- 247 ATCC Colorectal carcinoma Cell line (H. sapiens) OVCAR8 NIH:OVCAR8 Ovarian carcinoma Cell line (H. sapiens) DLBCL cell lines Dr. J. Moreaux Institute of Human Genetics, Montpellier Cell line (H. sapiens) SUM159 SUM159PT Asterand Bioscience Triple- negative breast cancer cell line Antibody Mouse monoclonal anti- BrdU clone B44 347580 BD Biosciences 1/100 Antibody Rat monoclonal anti- BrdU clone BU1/75 ABC117- 7513 Eurobio Abcys 1/100 Antibody Mouse monoclonal antissDNA MAB3868 Millipore 1/250 Antibody Rabbit monoclonal antipCHK1 (S345) 2348 Cell Signaling 1/1000 Antibody Rabbit polyclonal anti- pCHK2 (T68) 2661 Cell Signaling 1/1000 Antibody Mouse monoclonal anti-γH2AX (S139) 05- 636 Millipore 1/500 Antibody Mouse monoclonal anti- actin A4700 Sigma 1/500 Antibody Rabbit monoclonal anti- RPA1 Ab79398 Abcam 1/300 Coquel et al. eLife 2024;13:RP104718.

    Techniques: Incubation, Activity Assay, In Vitro, Phospho-proteomics, Western Blot, Control, Residue, Marker